Impact of insulin resistance on sperm parameters and serum osteocalcin levels: A prospective cross-sectional comparative study

Muserref B. Yilmaz, Ali Aras, Reyyan G. Iscan, Zeynep Celik, Halime H. Pence, Ayse N. Aksoy

Abstract

This prospective cross-sectional comparative study aimed to elucidate the effects of insulin resistance and the role of osteocalcin protein in male infertility. A cohort of 171 infertile men aged 25-55 was examined at an assisted reproduction clinic. Hormone profiles, glucose/insulin levels, osteocalcin concentrations, lipid profiles, and semen parameters were assessed. Using the HOMA index, patients were classified as insulin-resistant (n=87) or insulin-sensitive (n=84). Insulin-resistant patients had significantly lower total testosterone (p<0.001), free androgen index (p<0.001), and carboxylated osteocalcin levels (p=0.039) compared to insulin-sensitive patients. Linear regression analysis revealed a negative correlation between HOMA-IR and both total testosterone levels (p=0.022) and sperm motility (p=0.037). No association was found between HOMA-IR and osteocalcin levels (p=0.417). These findings suggest that insulin resistance negatively impacts male reproductive function, particularly testosterone levels and sperm motility. Larger studies are needed to confirm the role of osteocalcin in male infertility and its potential as a therapeutic target.

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References

Mehra BL, Skandhan KP, Prasad BS, Pawankumar G, Singh

G and Jaya V Male infertility rate: a retrospective

study. Urologia (2018) 85:22-4.

Zańko A, Siewko K, Krętowski AJ and Milewski R Lifestyle,

Insulin Resistance and Semen Quality as Co

Dependent Factors of Male Infertility. Int J Environ

Res Public Health (2022) 20:732.

Kasturi SS, Tannir J and Brannigan RE The metabolic

syndrome and male infertility.J Androl (2008) 29:251

Moser SC and van der Eerden BCJ Osteocalcin-A Versatile

Bone-Derived Hormone. Front Endocrinol (Lausanne)

(2018) 9:794.

Oury F, Sumara G, Sumara O, Ferron M, Chang H, Smith CE,

Hermo L, Suarez S, Roth BL, Ducy P and Karsenty G

Endocrine regulation of male infertility by the

skeleton.Cell (2011) 144:769–809.

Bilotta FL, Arcidiacono B, Messineo S, Greco M, Chiefari E,

Britti D, Nakanishi T, Foti DP and Brunetti A Insulin

and osteocalcin: further evidence for a mutual cross

talk. Endocrine. (2018) 59:622-632.

Mansour R, El-Faissal Y, Kamel A, Kamal O, Aboulserour

G, Aboulghar M and Fahmy I Increased insulin

resistance in men with unexplained infertility. Reprod

Biomed Online (2017) 35:571-575.

El-Kamshoushi AAM, Hassan EM and Hassaan PS

Evaluation of serum level of Osteocalcin hormone in

male infertility. Andrologia (2017) 49:10.1111-12755.

Saleh ES, Ameen IA and Taha KN Osteocalcin as a

Biomarker for Estimation of Infertility for Iraqi

Patients. International Journal of Drug Delivery

Technology (2020) 10:85-88.

De Toni L, Jawich K, De Rocco Ponce M, Di Nisio A and

Foresta C (2020) Osteocalcin: A Protein A Protein

Hormone Connecting Metabolism, Bone and Testis

Function. Protein Pept Lett 27:1268-1275.

Matthews DR, Hosker JP, Rudenski AS, Naylor BA,

Treacher DF and Turner RC Homeostasis model

assessment: insulin resistance and beta-cell function

from fasting plasma glucose and insulin concentrations in man. Diabetologia (1985) 28:412

Muniyappa R, Lee S, Chen H and Quon MJ Current

approaches for assessing insulin sensitivity and

resistance in vivo: advantages, limitations, and

appropriate usage. Am J Physiol Endocrinol Metab

(2008) 294:E15-E26.

Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker

HW, Behre HM, Haugen TB, Kruger T,Wang C,

Mbizvo MT, Vogelsong KM. World Health

Organization reference values for human seme

characteristics. Hum Reprod Update. 2010 May

Jun;16(3):231-45

Hamilton JM, Cissen M, Brandes M, Smeenk JMJ, de Bruin

JP and Kremer JM. Total motile sperm count: a better

indicator for the severity of male factor infertility than

the WHO sperm classification system. Hum Reprod.

; 30(5): 1110-21.

Hajder M, Hajder E, Husic A. The Effects of Total Motile

Sperm Count on Spontaneous Pregnancy Rate and

Pregnancy After IUI Treatment in Couples with Male

Factor and Unexplained Infertility. Med Arch. 2016

Feb;70(1):39-43.

Oury F, Ferron M, Huizhen W, Confavreux C, Xu L,

Lacombe J, Srinivas P, Chamouni A, Lugani

F, Lejeune H, Kumar TR, Plotton I and Karsenty G

.Osteocalcin regulates murine and human fertility

through a pancreas-bone-testis axis. J Clin Invest

(2013) 123:2421 2433.

Ma J, Han RY, Mei XA, Qi YN, Ma JY, Liu WJ and Wang

SS. Correlation of insulin resistance with male

reproductive hormone levels and semen parameters.

Zhonghua Nan Ke Xue. (2018) 24:695-699.

Shafaat P MS, Parvari S, Solhjo S, Tooli H, Nejad MY,

Dhulqarnain AO and Rastegar T Osteocalcin

Improved Spermatogenesis in Azoospermic Mouse

Model. T Open Science Journal (2018) 3:1-12.

Guedes JAC, Esteves JV, Morais MR, Zorn TM and Furuya

DT. Osteocalcin improves insulin resistance and

inflammation in obese mice: Participation of white

adipose tissue and bone. Bone. (2018) 115:68-82.

Wang Q, Zhang B, Xu Y, Xu H and Zhang N (2013) The

Relationship

between

Serum

Osteocalcin

Concentration and Glucose Metabolism in Patients

with Type 2 Diabetes Mellitus. Int J Endocrinol

:842598.

Babey ME, Ewing SK, Strotmeyer ES, Napoli N, Schafer

AL, Vittinghoff E, Gundberg CM and Schwartz AV

No

Evidence

of

Association

Between

Undercarboxylatedm Osteocalcin and Incident Type 2

Diabetes. J Bone Miner Res (2022) 37:876-884.

Choudhury AB, Sarkar PD, Sakalley DK and Petkar SB Role

of adiponectin in mediating the association of

osteocalcin with insulin resistance and type 2 diabetes:

a cross sectional study in pre- and post-menopausal

women. Arch Physiol Biochem (2014) 120:73-79.

Iki M, Tamaki J, Fujita Y, Kouda K, Yura A, Kadowaki E,

Sato Y, Moon JS, Tomioka K, Okamoto N

and Kurumatani N. Serum undercarboxylated

osteocalcin levels are inversely associated with

glycemic status and insulin resistance in an elderly

Japanese male population: Fujiwara-kyo Osteoporosis

Risk in Men (FORMEN) Study. Osteoporos Int (2012)

:761-770.

Karsenty G and Oury F Regulation of male fertility by the

bone-derived hormone osteocalcin. Mol Cell

Endocrinol (2014) 382:521-526

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