Effects of genistein supplementation on the characteristics of human sperm during liquid storage

Guangzhong Jiao, Huayu Lian, Ling Liu, Lili Chen, Luping Zhang, Xiaoyan Liu

Abstract

Cryopreservation, the most popular way to preserve human sperm, led to a significant decline in sperm motility. Here, we tried to introduce a new method to store sperm without freezing. Different concentrations of genistein were added to liquid preserved sperm. We investigated the effects of supplementation on sperm total antioxidative capacity (T-AOC), glutathione(GSH), methane dicarboxylic aldehyde (MDA), acrosomal enzyme activity, and fertilization ability of sperm. The effects of liquid storage and cryopreservation on sperm parameters were also compared. IVF medium supplemented with genistein (20µmol L-1 ) maintained sperm motility for up to 11 days. The addition of genistein led to a decrease in reactive oxygen species (ROS) generation that demonstrated an effective improvement in sperm motility and decreased the MDA production and maintained the GSH content and enhanced the oxidative stress resistance ability of the sperm during liquid storage. The storage sperm were used for intracytoplasmic sperm injection(ICSI) into human oocytes and activated oocytes successfully. Sperm stored in liquid medium containing genistein was superior to sperm stored in liquid nitrogen in terms of antioxidant stress and fertilization ability. We confirmed that genistein could be used as an antioxidant for the liquid storage of sperm. Sperm stored in an IVF medium with genistein could avoid cryodamage, which may become an alternative option in assisted reproduction technology. (Afr J Reprod Health 2022; 26[7]: 72- 82).

Full Text:

PDF

References

Oehninger S, Duru NK, Srisombut C and Morshedi M.

Assessment of sperm cryodamage and strategies to

improve outcome. Mol Cell Endocrinol 2000; 169:

–10.

O’Connell M, McClure N and Lewis SE. The effects of

cryopreservation on sperm morphology, motility and

mitochondrial function. Hum Reprod 2002; 17:

–9.

Schiller J, Arnhold J, Glander HJ and Arnold K. Lipid

analysis of human spermatozoa and seminal plasma

by MALDITOF mass spectrometry and NMR

spectroscopy–effects of freezing and thawing. Chem

Phys Lipids 2000; 106:145–56.

Critser JK, Arneson BW, Aaker DV, Huse-Benda AR and

Ball GD. Cryopreservation of human spermatozoa.

II. Postthaw chronology of motility and of zona-free

hamster ova penetration. Fertil Steril 1987; 47: 980–

Alvarez JG and Storey BT. Evidence that membrane stress

contributes more than lipid peroxidation to sublethal

cryodamage in cryopreserved human sperm: glycerol

and other polyols as sole cryoprotectant. J Androl

; 14: 199–209.

McLaughlin EA, Ford WC and Hull MG. The contribution

of the toxicity of a glycerol-egg yolk-citrate

cryopreservative to the decline in human sperm

motility during cryopreservation. J Reprod Fertil

; 95: 749–54.

Mack SR and Zaneveld LJ. Acrosomal enzymes and

ultrastructure of unfrozen and cryotreated human

spermatozoa. Gamete Res 1987; 18: 375–83.

Ono T, Mizutani E, Li C and Wakayama T. Preservation of

sperm within the mouse cauda epididymidis in salt or

sugars at room temperature. Zygote 2010; 18:245–

Bhowmick S, Zhu L, McGinnis L, Lawitts J, Nath BD,

Toner M and Biggers JD. Desiccation tolerance of

spermatozoa dried at ambient temperature:

production of fetal mice. Biol Reprod 2003;

:1779–1786.

McGinnis LK, Zhu L, Lawitts JA, Bhowmick S, Toner M

and Biggers JD. Mouse sperm desiccated and stored

in trehalose medium without freezing. Biol Reprod

; 73:627–633.

Jaskey DG, Cohen MR. Twenty-four to ninety-six-hour

storage of human spermatozoa in test-yolk buffer.

Fertil Steril 1981; 35:205–208.

Bolanos JR, Overstreet JW and Katz DF. Human sperm

penetration of zonafree hamster eggs after storage of

the semen for 48 hours at 2 degrees C to 5 degrees C.

Fertil Steril 1983; 39:536–541.

Kesseru E and Carrere C. Duration of vitality and

migrating ability of human spermatozoa

cryopreserved at þ4 degrees C. Andrologia 1984;

:429–433.

Martin RH, Templado C, Ko E and Rademaker A. Effect

of culture conditions and media on the frequency of

chromosomal abnormalities in human sperm

chromosome complements. Mol Reprod Dev 1990;

:101–104.

Munne S and Estop AM. Chromosome analysis of human

spermatozoa stored in vitro. Hum Reprod 1993;

:581–586.

Muratori M, Maggi M, Spinelli S, Filimberti E, Forti G and

Baldi E. Spontaneous DNA fragmentation in swimup selected human spermatozoa during long term

incubation. J Androl 2003; 24:253–262.

Schuffner A, Morshedi M, Vaamonde D, Duran EH and

Oehninger S. Effect of different incubation

conditions on phosphatidylserine externalization and

motion parameters of purified fractions of highly

motile human spermatozoa. J Androl 2002; 23:194–

Chatterjee S and Gagnon C. Production of reactive

oxygenspecies by spermatozoa undergoing cooling,

freezing, and thawing. Mol Reprod Dev 2001; 59:

–8.

Aitken RJ. The role of free oxygen radicals and sperm

function. Int J Androl 1989; 12: 95–7.

Alvarez JG and Storey BT. Differential incorporation of

fatty acids into and peroxidative loss of fatty acids

from phospholipids of human spermatozoa. Mol

Reprod Dev 1995; 42: 334–46.

White IG. Lipids and calcium uptake of sperm in relation

to cold shock and preservation: a review. Reprod

Fertil Dev 1993; 5: 639–58.

Aitken RJ. Free radicals, lipid peroxidation and sperm

function. Reprod Fertil Dev 1995; 7: 659–68.

Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe

S, Itoh N, Shibuya M and Fukami Y. Genistein, a

specifi inhibitor of tyrosine-specifi protein kinases. J

Biol Chem 1987; 262: 5592–5.

Sierens J, Hartley JA, Campbell MJ, Leathem AJ and

Woodside JV. Effect of phytoestrogen and

antioxidant supplementation on oxidative DNA

damage assessed using the comet assay. Mutat Res

; 485: 169–76.

Mitchell JH, Cawood E, Kinniburgh D, Provan A, Collins

AR and Irvine DS. Effect of a phytoestrogen food

supplement on reproductive health in normal males.

Clin Sci (Lond) 2001; 100: 613–8.

Sierens J, Hartley JA, Campbell MJ, Leathem AJ and

Woodside JV. In vitro isoflvone supplementation

reduces hydrogen peroxide-induced DNA damage in

sperm. Teratog Carcinog Mutagen 2002; 22: 227–34.

World Health Organization. WHO Laboratory Manual for

the Examination of Human Semen and SpermCervical Mucus interaction, 4th ed. Cambridge:

Cambridge University Press; 1999. p128.

Stradaioli G, Noro T, Sylla L and Monaci M. Decrease in

glutathione (GSH) content in bovine sperm after

cryopreservation: comparison between two

extenders. Theriogenology 2007; 67:1249-1255.

Gadea J, Selles E, Marco MA, Coy P, Matas C, Romar R

and Ruiz S. Decrease in glutathione content in boar

sperm after cryopreservation. Effect of the addition

of reduced glutathione to the freezing and thawing

extenders. Theriogenology 2004; 62: 690-701.

Quan S, Zhou HK, Shuji Y, Hisayo N and Toshihiro A.

Fertilizing capacity of human sperm preserved in

cold electrolyte-free solution. Di Yi Jun Yi Da Xue

Xue Bao 2002; 22:928–930.

Sakkas D and Alvarez JG. Sperm DNA fragmentation:

mechanisms of origin, impact on reproductive

outcome, and analysis. Fertil Steril 2010; 93:1027–

Aitken RJ and Koppers AJ. Apoptosis and DNA damage

in human spermatozoa. Asian J Androl 2011; 13:36–

Gomez E, Buckingham DW, Brindle J, Lanzafame F,

Irvine DS and Aitken RJ. Development of an image

analysis system to monitor the retention of residual cytoplasm by human spermatozoa: correlation with

biochemical markers of the cytoplasmic space,

oxidative stress, and sperm function. J Androl 1996;

: 276–87.

Aitken RJ, De Iuliis GN and McLachlan RI. Biological and

clinicalsignificance of DNA damage in the male

germ line. Int J Androl 2009; 32:46–56.

Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P,

Jennings Z and Irvine DS. Relative impact of

oxidative stress on the functional competence

andgenomic integrity of human spermatozoa. Biol

Reprod 1998; 59:1037–1046.

Bajpai M, Asin S and Doncel GF. Effect of tyrosine kinase

inhibitors on tyrosine phosphorylation and motility

parameters in human sperm. Arch Androl 2003; 49:

–46.

Kumi-Diaka J and Townsend J. Effects of genistein

isoflvone(4′,5 ′,7-trihydroxyisoflavone) and

dexamethasone on functional characteristics of

spermatozoa. J Med Food 2001; 4: 39–47.

Bajpai M and Doncel GF. Involvement of tyrosine kinase

and cAMP-dependent kinase cross-talk in the

regulation of human sperm motility. Reproduction

; 126: 183–95.

Shi Z, Lv Z, Hu C, Zhang Q, Wang Z, Hamdard E, Dai H,

Mustafa S and Shi F. Oral Exposure to Genistein

during Conception and Lactation Period Affects the

Testicular Development of Male Offspring Mice.

Animals (Basel) 2020; 10(3):377.

Rago V, Romeo F, Giordano F, Malivindi R, Pezzi V,

Casaburi I and Carpino A. Expression of oestrogen

receptors (GPER, ESR 1, ESR 2) in human ductuli

efferentes and proximal epididymis. Andrology 2018

；6:192–198.

Coban N, Gulec C, Ozsait-Selcuk B and Erginel-Unaltuna

N. CYP19A1, MIF and ABCA1 genes are targets of

the RORα in monocyte and endothelial cells. Cell

Biol Int 2017；1:163–176.

Nakamoto M, Shibata Y, Ohno K, Usami T, Kamei Y,

Taniguchi Y, Todo T, Sakamoto T, Young G,

Swanson P, Naruse K and Nagahama Y. Ovarian

aromatase loss-of-function mutant medaka undergo

ovary degeneration and partial female-to-male sex

reversal after puberty. Mol Cell Endocrinol 2018；

:104–122.

Wang H, Zhao R, Guo C, Jiang S, Yang J, Xu Y, Liu Y,

Fan L, Xiong W, Ma J, Peng S, Zeng Z, Zhou Y, Li

X, Li Z, Li X, Schmitt DC, Tan M, Li G and Zhou

M. Knockout of BRD7 results in impaired

spermatogenesis and male infertility. Sci Rep 2016

；6:1–13.

Swart AC, Johannes ID, Sathyapalan T and Atkin SL. The

Effect of Soy Isoflavones on Steroid Metabolism.

Front Endocrinol 2019；10: 229.

Mukund V, Mukund D, Sharma V, Mannarapu M and

Alam A. Genistein: Its role in metabolic diseases and

cancer. Crit Rev Oncol Hematol 2017；119: 13–22.

De Gregorio C, Marini H, Alibrandi A, Di Benedetto A,

Bitto A, Adamo EB, Altaville D, Irace C, Vieste GD,

Pancaldo D, Granese R, Atteritano M, Corrao S,

Licata G, Squadrito F and Arcoraci V. Genistein

supplementation and cardiac function in

postmenopausal women with metabolic syndrome:

Results from a pilot strain-echo study. Nutrients

；9: 584.

Zribi N, Feki Chakroun N, El Euch H, Gargouri J, Bahloul

A and Ammar Keskes L. Effects of cryopreservation

on human sperm deoxyribonucleic acid integrity.

Fertil Steril 2010; 93:159–166.

Toro E, Fernandez S, Colomar A, Casanovas A, Alvarez

JG, Lopez-Teijon ML and Velilla E. Processing of

semen can result in increased sperm DNA

fragmentation. Fertil Steril 2009; 92:2109–2112.

Glander HJ and Schaller J. Hidden effects of

cryopreservation on quality of human spermatozoa.

Cell Tissue Bank 2000; 1:133–142.

Simon L, Brunborg G, Stevenson M, Lutton D, McManus

J and Lewis SE. Clinical significance of sperm DNA

damage in assisted reproduction outcome. Hum

Reprod 2010; 25:1594–1608.

Riel JM, Huang TT and Ward MA. Freezing-free

preservation of human spermatozoa—a pilot study.

Arch Androl 2007; 53:275–284.

Lewis SE, Sterling ES, Young IS and Thompson W.

Comparison of individual antioxidants of sperm and

seminal plasma in fertile and infertile men. Fertil

Steril 1997; 67:142–147.

Mann T and Lutwak-Mann C. Male Reproductive Function

and Semen. Berlin: Springer-Verlag; 1981

Refbacks

  • There are currently no refbacks.