Global sperm DNA methylation and intra cytoplasmic sperm injection outcomes

Ossama H. Roshdy, Rania E. Abdel Maksoud, Yasser S. El Kassar, Doreen N. Younan, Rana R. El Sayed

Abstract

Routine semen analysis is a poor predictor of pregnancy rates after intra-cytoplasmic sperm injection (ICSI). There is an assumption that embryos’ quality and fertilization rates could be influenced by epigenetic factors. We aimed at comparing global sperm DNA methylation level (GSDML) between normal and abnormal semen, investigating its relationship with sperm parameters and assessing its effect on ICSI outcomes; fertilization, good embryo and pregnancy rates. Ejaculates were obtained from 104 infertile and 60 fertile males undergoing ICSI at Faculty of Medicine, Alexandria, Egypt. Data was analyzed using IBM SPSS software package, 20. Inter-group differences in semen parameters were assessed by t-test. GSDML, measured by ELISA, showed significant positive correlation with sperm count, concentration and motility. It correlated positively but insignificantly with morphology and fertilization rate. High levels were significantly associated with embryos having good quality and positive pregnancy rates. GSDML could predict good embryo rate and pregnancy occurrence after ICSI. (Afr J Reprod Health 2020; 24[4]: 94-100).

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References

Ford WC. Comments on the release of the 5th edition of

the WHO laboratory manual for the examination and processing of human semen. Asian J Androl 2010; 12(1):59- 63. https://doi: 10.1038/aja.2008.57.

Sharma U. Paternal Contributions to Offspring Health:

Role of Sperm Small RNAs in Intergenerational Transmission of Epigenetic Information. Front Cell Dev Biol 2019; 7:215. https:// doi: 10.3389/fcell.2019.00215.

Colaco S and Sakkas D.

Paternal factors contributing to embryo quality. J Assist Reprod Genet 2018; 35(11): 1953–68. https:// doi: 10.1007/s10815-018-1304-4.

Sun TC, Zhang Y, Li HT, Liu XM, Yi DX, Tian L

and Liu YX. Sperm DNA fragmentation index, as measured by sperm chromatin dispersion, might not predict assisted reproductive outcome. Taiwan J Obstet Gynecol 2018; 57(4):493-8. https://doi: 10.1016/j.tjog.2018.06.003.

Dogra S, Sona C, Kumar A and Yadav PN.

Epigenetic regulation of G protein coupled receptor signaling and its implications in psychiatric disorders. Int J Biochem Cell Biol 2016; 77(Pt B):226- 39. https://doi: 10.1016/j.biocel.2016.03.012. Epub 2016 Mar 29.

Katib AA, Al-Hawsawi K, Motair W and Bawa AM.

Secondary infertility and the aging male, overview. Cent European J Urol 2014; 67(2):184-8. https://doi: 10.5173/ceju.2014.02.art13. Epub 2014 Jun 23.

Skinner MK. Environmental epigenomics and disease

susceptibility. EMBO Rep 2011; 12(7):620- 2. https://doi: 10.1038/embor.2011.125.

Raad G, Azouri J, Rizk K, Zeidan NS, Azouri J,

Grandjean V and Hazzouri M. Adverse effects of paternal obesity on the motile spermatozoa quality. PLoS One. 2019; 14(2): e0211837. Published online 2019 Feb 11. doi: 10.1371/journal.pone.0211837

Marques CJ, Costa P, Vaz B, Carvalho F, Fernandes

S, Barros A and Sousa M. Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermia. Mol Hum Reprod 2008; 14(2):67- 74. https://doi: 10.1093/molehr/gam093. Epub 2008 Jan 4.

Montjean D, Ravel C, Benkhalifa M, Cohen-Bacrie

P, Berthaut I, Bashamboo A and McElreavey K. Methylation changes in mature sperm deoxyribonucleic acid from oligozoospermic men: assessment of genetic variants and assisted reproductive technology outcome. Fertil Steril 2013; 100(5):1241-7. https://doi: 10.1016/j.fertnstert.2013.06.047. Epub 2013 Aug 2.

Benchaib M, Braun V, Ressnikof D, Lornage J, Durand

P, Niveleau A and Guérin JF. Influence of global sperm DNA methylation on IVF results. Hum Reprod 2005; 20(3):768- 73. Epub 2005 Jan 7.

Ebner T, Yaman C, Moser M, Sommergruber M, Pölz W

and Tews G. Embryo fragmentation in vitro and its impact on treatment and pregnancy outcome. Fertil Steril 2001; 76(2):281-5.

Binu VS, Mayya SS and Dhar M. Some basic aspects of

statistical methods and sample size determination in health science research. Ayu 2014; 35(2):119-23.

Marques CJ, Carvalho F, Sousa M and Barros A.

Genomic imprinting in disruptive spermatogenesis. Lancet 2004; 363(9422):1700-2.

Houshdaran S, Cortessis VK, Siegmund K, Yang

A, Laird PW and Sokol RZ. Widespread epigenetic abnormalities suggest a broad DNAmethylation erasure defect in abnormal human sperm. PLoS One 2007; 2(12):e1289.

El Hajj N, Zechner U, Schneider E, Tresch A, Gromoll

J, Hahn T, Schorsch M and Haaf T. Methylation status of imprinted genes and repetitive elements in sperm DNA from infertile males. Sex Dev 2011; 5(2):60-9.https:// doi: 10.1159/000323806. Epub 2011 Feb 4.

Kobayashi H, Sato A, Otsu E, Hiura H, Tomatsu C,

Utsunomiya T, Sasaki H, Yaegashi N and Arima T. Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum Mol Genet 2007; 16(21):2542-51. Epub 2007 Jul 17.

Choufani S, Turinsky AL, Melamed N, Greenblatt E,

Brudno M, Bérard A, Fraser WD, Weksberg R, Trasler J and Monnier P. 3D cohort study group. Impact of assisted reproduction, infertility, sex and paternal factors on the placental DNA methylome. Hum Mol Genet 2019; 28(3): 372–85. https:// doi: 10.1093/hmg/ddy321.

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